Incidence and Molecular Identification of Escherichia Coli Harbouring Gentamicin Resistant Gene among Pregnant Women

Tawfeeq, Hassan M. and Tofiq, Ahmed M. and Ali, Kameran M. (2017) Incidence and Molecular Identification of Escherichia Coli Harbouring Gentamicin Resistant Gene among Pregnant Women. Eurasian Journal of Science and Engineering, 3 (1). pp. 117-127. ISSN 24145629

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Abstract

Pregnant-origin Gram negative enteric bacteria, particularly, Escherichia coli isolates were exposed to tests for resistance phenotype using Kirby-Bauer’s disk-diffusion assay and for gentamicin resistance genotype using aacC2gene, codifying for one specific aminoglycoside-modifying enzyme. The incidence of Enterobacteriaceae was 31 (21.8%), emerged from142 midstream urine (MSU) samples, including symptomatic (17.6%) and asymptomatic (25.7%) bacteriuria. Women with age brackets from 15-44 years were studied. Age group 25-29 years scored the highest incidence rate of infection (29.2%), while age group 35-39 years exhibited the lowest incidence rate. Data described previously were statistically insignificant (P value > 0.05). Remarkably, significant differences (P value <0.05) observed concerning gestational ages when third (32.81%) and second (14.81%) trimesters were noticed with the highest rate of bacteriuria, respectively. E. coli distinguished as the most predominant (41.9%), followed by Klebssiella pneumoniae (19.4%) and Pseudomonas aeruginosa (12.9%), consecutively. Seven other uropathogens were identified with a lesser frequency. Isolates subjected to numerous antibiotics and results revealed fully resistance to pencillin G and amoxicillin-clavulanic acid, while (96.8%) possessed resistant to amoxicillin, cephlexin, and vancomycin. However, amikacin still acting perfectly (90.3%) against all the isolates, especially towards E. coli (92.3%). Concurrently, the antimicrobial potency of streptomycin, ciprofloxacin, and ceftriaxone were (92.3%), (84.6%), and (77%) respectively, when assayed opposite E. coli strains solely. Phenotypically, (30.8%) of E. coli strains, which stand for about (32.3%) of the whole isolates, were resistance to gentamicin. 75% of phenotypically gentamicin resistance E. coli demonstrated genotypically as harbouring the aacC2 gene.

Item Type: Article
Uncontrolled Keywords: E. Coli, UTI, Pregnancy, Aacc2 Gene, Antibiogram
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Ishik Eurasian Journal of Science and Engineering > VOL 3, NO 1 (2017)
Depositing User: Depositor @ Ishik University
Date Deposited: 23 Apr 2018 20:53
Last Modified: 23 Apr 2018 20:53
URI: http://eprints.ishik.edu.iq/id/eprint/40

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